Dairy Farm Age and Resistance to Antimicrobial Agents in <Emphasis Type="Italic">Escherichia coli</Emphasis> Isolated from Dairy Topsoil |
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| Authors: | Suzanna E Jones Jonathan M Burgos Marvin M F Lutnesky Johnny A Sena Sanath Kumar Lindsay M Jones Manuel F Varela |
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| Institution: | (1) Department of Biology, Eastern New Mexico University, Roosevelt Hall, Room 101, Station 33, Portales, NM 88130, USA;(2) Present address: Laboratory of Respiratory and Special Pathogens, Division of Bacterial, Parasitic, and Allergenic Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA;(3) Present address: Department of Molecular Biology, University of Colorado Health Sciences, Anschutz Medical Campus, 12631 E. 17th Ave, Aurora, CO 80045, USA; |
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| Abstract: | Antimicrobial agent usage is common in animal agriculture for therapeutic and prophylactic purposes. Selective pressure exerted
by these antimicrobials on soil bacteria could result in the selection of strains that are resistant due to chromosomal- or
plasmid-derived genetic components. Multiple antimicrobial resistances in Escherichia coli and the direct relationship between antimicrobial agent use over time has been extensively studied, yet the relationship
between the age of an animal agriculture environment such as a dairy farm and antibiotic resistance remains unclear. Therefore,
we tested the hypothesis that antimicrobial-resistance profiles of E. coli isolated from dairy farm topsoil correlate with dairy farm age. E. coli isolated from eleven dairy farms of varying ages within Roosevelt County, NM were used for MIC determinations to chloramphenicol,
nalidixic acid, penicillin, tetracycline, ampicillin, amoxicillin/clavulanic acid, gentamicin, trimethoprim/sulfamethoxazole,
cefotaxime, and ciprofloxacin. The minimum inhibitory concentration values of four antibiotics ranged 0.75 to >256 μg/ml,
1 to >256 μg/ml, 12 to >256 μg/ml, and 0.75 to >256 μg/ml for chloramphenicol, nalidixic acid, penicillin, and tetracycline,
respectively. The study did not show a direct relationship between antibiotic resistance and the age of dairy farms. |
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