Optimization of somatic cell injection in the perspective of nuclear transfer in goldfish |
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Authors: | Pierre-Yves Le Bail Alexandra Depince Nathalie Chenais Sophie Mahe Gerard Maisse Catherine Labbe |
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Institution: | 1.INRA, Cryopreservation and Regeneration of Fish, UR1037 SCRIBE,Rennes,France;2.AFSSA-LEREPP, Unité de Virologie Immunologie Porcines,Ploufragan,France |
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Abstract: | Background Nuclear transfer has the potential to become one strategy for fish genetic resources management, by allowing fish reconstruction
from cryopreserved somatic cells. Survival rates after nuclear transfer are still low however. The part played by unsuitable
handling conditions is often questioned, but the different steps in the procedure are difficult to address separately. In
this work led on goldfish (Carassius auratus), the step of somatic cells injection was explored. Non-enucleated metaphase II oocytes were used as a template to explore
the toxicity of the injection medium, to estimate the best location where the cell should be injected, and to assess the delay
necessary between cell injection and oocyte activation. |
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Keywords: | |
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