The Effects of Hypoxia/Reoxygenation on the Physiological Behaviour of U373-Mg Astrocytes |
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Authors: | Carlo Aldinucci Silvia Maria Maiorca Paola De Rosa Mitri Palmi Claudia Sticozzi Lucia Ciccoli Silvia Leoncini Cinzia Signorini Gian Paolo Pessina |
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Institution: | (1) Department of Physiology, University of Siena, via A. Moro, 53100 Siena, Italy;(2) Department of Biomedical Sciences, University of Siena, 53100 Siena, Italy;(3) Department of Pathophysiology, Experimental Medicine and Public Health, University of Siena, 53100 Siena, Italy; |
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Abstract: | Nerve cells are very susceptible to hypoxia responsive for mitochondrial dysfunctions involved in the subsequent oxidative
stress, apoptosis and necrosis. In this paper, we examined the effect of 12 h incubation of U-373 MG astrocytes in hypoxic
environment (73% N2: 2% O2: 5% CO2, v:v) by evaluating cell proliferation, modifications of NO and ATP production, intracellular Ca2+ concentration Ca2+]i, membrane potential, desferoxamine-chelatable free iron, esterified F2-isoprostanes levels and the production of phosphorylated
ERK. The same parameters were evaluated also after a following re-oxygenation period of 24 h. Immediately after hypoxia the
NO concentration increased significantly and returned to values similar to those of controls after the re-oxygenation period.
At the same time, ATP levels remained similar to controls and the cell proliferation significantly decreased. This involved
a significant increase of Ca2+]i immediately after hypoxia and the value remained significantly elevated after the following re-oxygenation period. Moreover,
after hypoxia, astrocytes were slightly although not significantly depolarized. Indeed iron and F2-isoprostanes levels increased
significantly after hypoxia. Finally ERK proteins increased slowly and not significantly after hypoxia and the same trend
was observed after the re-oxygenation period. On the whole, our results indicate that 2% O2 hypoxia induces a moderate oxidative stress, well tolerated by U-373 MG cells, remaining the ATP production, mitochondrial
membrane potential and activated ERK proteins, similar to the values of controls. |
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