DNA sequence of the araBAD-araC controlling region in Salmonella typhimurium LT2 |
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Authors: | Arnold H. Horwitz Laurel Heffernan Carlo Morandi Jar-How Lee Josef Timko Gary Wilcox |
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Affiliation: | Department of Microbiology and Molecular Biology Institute, University of California, Los Angeles, CA 99024 U.S.A. |
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Abstract: | The araB and araC genes of Salmonella typhimurium have been cloned onto the plasmid pBR322. Restriction analysis and subcloning of restriction fragments localized these genes to a 4.4 kb DNA fragment. Complementation analysis revealed that the cloned araB and araC genes from S. typhimurium complemented araB and araC mutant strains of Escherichia coli. Conversely, cloned araB and araC genes from E. coli complemented araB and araC mutant strains of S. typhimurium. The DNA sequences was determined for the S. typhimurium araB and araC controlling region and for the initially translated portions of these genes. The nucleotide sequence of the araB promoter was 87% homologous with the same region in E. coli and contained no deletions or insertions relative to the E. coli sequence. The presumed AUG codon corresponding to the amino terminus of the S. typhimurium araC protein was in the same location as in E. coli. There was, however, considerable divergence from the E. coli sequence preceding the translation start site. The nucleotide sequence of the initial 237 bp in the open reading frame of the S. typhimurium araC gene was 78% homologous with the same sequence in E. coli. By comparison, the amino acid sequence for this region was 91% conserved. |
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Keywords: | cloning restriction mapping complementation analysis ampicillin-resistant bp base pairs cAMP adenosine 3′:5′-cyclic monophosphate CRP cAMP receptor protein kb kilobase pairs pfu plaque-forming units tetracycline-sensitive :: novel joint |
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