Restriction endonuclease analysis of DNA from the Streptomyces phages SH3, SH5, SH10 and SH13 |
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| Authors: | Friedrich Walter Manfred Hartmann Siegfried Klaus |
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| Institution: | Akademie der Wissenschaften der DDR, Forschungszentrum für Molekularbiologie und Medizin, Zentralinstitut für Mikrobiologie und experimentelle Therapie, Abteilungen Molekulare Biochemie und Mikrobengenetik, DDR-69 Jena, Beutenbergstrasse 11 G.D.R. |
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| Abstract: | Using 26 restriction endonucleases, a cleavage site survey was undertaken for DNAs of several unrelated Streptomyces phages SH3, SH5, SH10 and SH13. Only EcoRI was found to produce single cleavage in SH3 and SH10 DNA. The complete maps were prepared for the 2, 9 and 11 fragments of SH10 DNA, as generated by EcoRI, KpnI and BglII, respectively. The evidence is presented that SH10 DNA contains cohesive ends. Moreover, a clearplaque mutant of SH10 was shown to contain a deletion of 790 bp in the right part of the genome, including two KpnI sites. |
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| Keywords: | Actinophages gel electrophoresis cohesive ends physical maps deletion mutant bp base pairs Md megadaltons pfu plaqueforming units SDS sodium dodecyl sulfate |
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