Optimization of immunomagnetic separation for cord blood-derived hematopoietic stem cells |
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Authors: | Tuija Kekarainen Sirkka Mannelin Jarmo Laine Taina Jaatinen |
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Affiliation: | 1. Finnish Red Cross Blood Service, Helsinki, Finland 2. Centre de Recerca en Sanitat Animal (CReSA), Universitat Autònoma de Barcelona, Barcelona, Spain
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Abstract: | Background There is a growing interest in cord blood as a source of primitive stem cells with the capacity for multilineage differentiation. Pure cell fractions are needed for the characterization and in vitro expansion of stem cells as well as for their use in preclinical research. However, enrichment of stem cells is challenging due to the lack of stem cell-specific markers and gentle protocols for the isolation of highly pure stem cell fractions. Protocols developed for the enrichment of peripheral blood-derived stem cells have been found to be suboptimal for cord blood. |
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