Production of hepatitis B core antigen in a stirred tank bioreactor: The influence of temperature and agitation |
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Authors: | Beng Ti Tey Mung Ing Chua Ghee Sung Chua Michelle Yeen Tan Ng Dayang Radiah Awang Biak Wen Siang Tan Tau Chuan Ling |
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Abstract: | The influence of temperature and agitation on the growth ofEscherichia coli expressing hepatitis B core antigen (HBcAg) in stirred tank bioreactor were investigated. The highest specific growth rate forE. coli (0.844 h−1) was achieved at a temperature of 37°C and an agitation speed of 250 rpm. The activation energy for the growth of theE. coli strain W3110IQ in the stirred tank bioreactor was estimated to be 11 kcal/mol. The highest protein yield was achieved at a temperature of 44°C and an agitation speed of 250 rpm. The relative protein concentration at 44°C is 30 and 6% higher compared to that at 30 and 37°C, respectively. |
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