Development of a new set of reference genes for normalization of real-time RT-PCR data of porcine backfat and longissimus dorsi muscle, and evaluation with PPARGC1A |
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Authors: | Tim Erkens Mario Van Poucke Jo Vandesompele Karen Goossens Alex Van Zeveren and Luc J Peelman |
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Institution: | (1) Department of Nutrition, Genetics and Ethology, Faculty of Veterinary Medicine, Ghent University, Heidestraat 19, 9820 Merelbeke, Belgium;(2) Centre for Medical Genetics, Ghent University Hospital, De Pintelaan 185, 9000 Ghent, Belgium |
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Abstract: | Background An essential part of using real-time RT-PCR is that expression results have to be normalized before any conclusions can be
drawn. This can be done by using one or multiple, validated reference genes, depending on the desired accuracy of the results.
In the pig however, very little information is available on the expression stability of reference genes. The aim of this study
was therefore to develop a new set of reference genes which can be used for normalization of mRNA expression data of genes
expressed in porcine backfat and longissimus dorsi muscle, both representing an economically important part of a pig's carcass. Because of its multiple functions in fat metabolism
and muscle fibre type composition, peroxisome proliferative activated receptor γ coactivator 1α (PPARGC1A) is a very interesting candidate gene for meat quality, and was an ideal gene to evaluate our developed set of reference
genes for normalization of mRNA expression data of both tissue types. |
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