A novel strategy using MASCOT Distiller for analysis of cleavable isotope-coded affinity tag data to quantify protein changes in plasma |
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Authors: | Leung Kit-Yi Lescuyer Pierre Campbell James Byers Helen L Allard Laure Sanchez Jean-Charles Ward Malcolm A |
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Institution: | Proteome Sciences plc, South Wing Laboratory, Institute of Psychiatry, London, UK. kit-yi.leung@proteomics.com |
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Abstract: | A novel strategy consisting of cleavable Isotope-Coded Affinity Tag (cICAT) combined with MASCOT Distiller was evaluated as a tool for the quantification of proteins in "abnormal" patient plasma, prepared by pooling samples from patients with acute stroke. Quantification of all light and heavy cICAT-labelled peptide ion pairs was obtained using MASCOT Distiller combined with a proprietary software. Peptides displaying differences were selected for identification by MS. These preliminary results show the promise of our approach to identify potential biomarkers. |
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