Heterologous expression of lignin peroxidase of Phanerochaete chrysosporium in Pichia methanolica |
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| Authors: | Haikuan Wang Fuping Lu Yafan Sun Lianxiang Du |
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| Affiliation: | Department of Food Science and Biology Engineering, Tianjin Science and Technology University, P.R. China. |
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| Abstract: | The cDNA encoding for lignin peroxidase of Phanerochaete chrysosporium was expressed in the Pichia methanolica under the control of the alcohol oxidase (AUG1) promoter which was followed by either the lignin peroxidase leader peptide of Phanerochaete chrysosporium or the Saccharomyces cerevisiae alpha-factor signal peptide. Both peptides efficiently directed the secretion of lignin peroxidase from the recombinant yeast cell. The extracellular lignin peroxidase activity in two recombinants was 932 U l(-1) and 1933 U l(-1). The purity of the recombinant product was confirmed by SDS-PAGE. |
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| Keywords: | heterologous expression lignin peroxidase Phanerochaete chrysosporium Pichia methanolica |
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