Improved production of recombinant rotavirus VP6 in sodium butyrate-supplemented suspension cultures of transgenic tomato (Lycopersicon esculentum Mill.) cells |
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| Authors: | Chang Heon Kim Kyung Il Kim Seong Hyun Hong Youn Hyung Lee In Sik Chung |
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| Affiliation: | (1) Department of Genetic Engineering, Kyung Hee University, Suwon, 449-701, Korea;(2) Present address: Plant Biotechnology Center, Ohio State University, Columbus, OH 43210, USA |
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| Abstract: | Recombinant rotavirus VP6 production was 0.49 mg l–1 when 10 mM sodium butyrate was added to the suspension culture of transgenic tomato cells after 6 days' incubation. Recombinant VP6 production increased to 0.73 mg l–1, when the culture was supplemented with 10 mM sodium butyrate on day 6 and 9 after incubation. Total protein extracts of transgenic tomato cells from sodium butyrate-supplemented culture were examined by two-dimensional electrophoresis: 14 proteins were up-regulated and 7 down-regulated. |
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| Keywords: | 2-D PAGE proteomics rotavirus VP6 sodium butyrate transgenic tomato cells |
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