| 中国板栗EST-SSR分子标记在栲树中的通用性分析 |
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| 引用本文： | 李春,孙晔. 中国板栗EST-SSR分子标记在栲树中的通用性分析[J]. 广西植物, 2012, 32(3): 293-297 |
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| 作者姓名： | 李春 孙晔 |
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| 作者单位： | 李春 (中国科学院华南植物园,广州510650) ; 孙晔 (中国科学院研究生院,北京100049) ; |
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| 基金项目： | 国家自然科学基金,中国科学院生命科学领域基础前沿研究专项,大科学装置工程项目 |
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| 摘 要： | 简单重复序列也称为微卫星分子标记,不仅在同属近缘种间具有良好的通用性,甚至在近缘属间也具有一定的通用性。本研究利用壳斗科基因组信息数据库中公布的中国板栗124对多态的EST-SSR引物在栲树中进行跨属(栗属到栲属)通用性研究,结果显示中国板栗EST-SSR引物在栲树中通用性和多态性分别为42.7%和56.6%;使用19对多态的EST-SSR引物对4个栲树自然居群的遗传多样性进行初步分析,结果显示栲树自然居群具有较高的遗传多样性(Na=6.105,Ho=0.563,He=0.621)。这些引物为栲树群体遗传学的深入研究提供了有力工具。
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| 关 键 词： | 中国板栗 栲树 EST-SSR 通用性 |
Transferabilityanalysis of EST-SSR markers of Castanea mollissimato Castanopsis fargesii |
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| LI Chun,SUN Ye. Transferabilityanalysis of EST-SSR markers of Castanea mollissimato Castanopsis fargesii[J]. Guihaia, 2012, 32(3): 293-297 |
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| Authors: | LI Chun SUN Ye |
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| Affiliation: | 1,3*(1.South China Botanical Garden,Chinese Academy of Sciences,Guangzhou 510650,China ;2.Graduate School of Chinese Academy of Sciences,Beijing 100049,China ;3.Key Laboratory of Plant Resources Conservation and Sustainable Utilization,South China Botanical Garden,Chinese Academy of Sciences,Guangzhou 510650,China) |
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| Abstract: | Simple sequence repeats(SSR),also known as microsatellite molecular markers,have been cross-amplified successfully in closely related species of the same genus and even across genera within the same family.In the present study,124primer pairs of polymorphic EST-SSR originally developed fromCastanea mollissima were cross-amplified in Castanopsis fargesii.Results indicated that 42.7% of Castanea mollssima EST-SSR primers were successfully cross-amplified in Castanopsis fargesii and 56.6% were polymorphic.The genetic diversity of 4populations of C.fargesii were investigated with polymorphic EST-SSRs,preliminary results showed that C.fargesii possessed high levels of genetic diversity(Na=6.105,Ho=0.563,He=0.621).These polymorphic EST-SSR primers would provide a powerful tool for further investigation on population genetics of C.fargesii. |
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| Keywords: | Castanea mollissima Castanopsis fargesii EST-SSR transferability |
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