An EF-hands protein, centrin-1, is an EGTA-sensitive SUMO-interacting protein in mouse testis |
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Authors: | Tanaka Niina Goto Miki Kawasaki Azusa Sasano Takashi Eto Ko Nishi Ryotaro Sugasawa Kaoru Abe Shinichi Saitoh Hisato |
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Institution: | Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University, Kumamoto, Japan. |
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Abstract: | A multifunctional calcium‐binding protein, centrin‐1, is specifically expressed in male germ cells, certain neurons and ciliated cells. We identified centrin‐1 as a protein interacting with SUMO‐2/3 using yeast two‐hybrid screening of a mouse testicular cDNA library. In bead halo assays, the interaction between centrin‐1 and SUMO‐2/3 was reduced in the presence of EGTA and facilitated by the addition of CaCl2. immunostaining of seminiferous tubules in 35‐day‐old mouse testes revealed that cells in the layer containing spermatogonia showed colocalization of SUMO‐2/3 with centrin‐1 in cytoplasmic spots. Identification of centrin‐1 as the EGTA‐sensitive SUMO‐2/3‐interacting protein indicates the possible role of calcium in modulating the centrin‐1–SUMO‐2/3 interaction and suggests the importance of this interaction in mouse testis. Copyright © 2010 John Wiley & Sons, Ltd. |
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Keywords: | SUMO‐2/3 centrin‐1 calcium signaling spermatogenesis |
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