A new method to induce sclerotial differentiation in Polyporus umbellatus by split-plate culture |
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Authors: | W-Q Yin S-X Guo Y-M Xing X-K Xing |
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Institution: | 1. Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, No. 151, Malianwa North Road, Haidian District, Beijing, 100193, China
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Abstract: | Polyporus umbellatus is one of the most valuable medicinal fungi, and its sclerotium has been used as a diuretic agent and an antidote in traditional Chinese medicine. In nature, Polyporus umbellatus has almost been depleted because of over-exploitation and lack of natural habitats. Thus, artificial sclerotia production has increased. This study aimed at finding an effective method to induce sclerotia, and selected the split-plate culture method. One side contained fructose agar medium (FAM), while the other side contained nutrient-limited medium. It was observed that sclerotia were only formed on the nutrient-limited medium side but scarcely emerged on the FAM side, even when the fructose concentration on both sides were the same. The sclerotial differentiation rate was 100% and the sclerotial yield was 106% higher than in the conventional way. In conclusion, the split-plate culture method is an effective way to induce P. umbellatus sclerotia in the laboratory. |
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