In vitro flowering of indica rice (Oryza sativa L. spp. indica) |
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Authors: | Suriyan Cha-um Thapanee Samphumphuang Chalermpol Kirdmanee |
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Institution: | (1) National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park, Paholyothin Road, Klong 1, Klong Luang, 12120, Pathumthani, Thailand |
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Abstract: | Nodal explants of rice cultivar Pathumthani 1 (PT1; short-day photoperiod insensitive) were collected, surface-disinfected,
and cultured on modified MS medium under in vitro conditions for 90 d. A total of 60% nodal explants generated flowering plantlets (with one inflorescence per cluster). The
net photosynthetic rate was greater, and soluble sugars (including glucose, fructose, and sucrose) accumulated to higher levels
in the leaves of flowering as compared to non-flowering plants. In contrast, chlorophyll a, chlorophyll b, total chlorophyll,
and total carotenoid content were enriched to a greater degree in the leaves of non-flowering as compared to flowering plants.
Also, growth performance parameters, including plant height, number of leaves per plant, leaf area, fresh weight, and dry
weight of plantlets derived from seedlings were superior to those of plantlets derived from nodal explants. In addition, the
protocol proved to successfully induce flowering in KDML 105, a short-day photoperiod-sensitive rice cultivar. |
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