<Emphasis Type="Italic">In vitro</Emphasis> regeneration of plants from sugarcane seed-derived callus |
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Authors: | K?Chengalrayan A?Abouzid Email author" target="_blank">M?Gallo-MeacherEmail author |
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Institution: | (1) Agronomy Department, University of Florida, 2183 McGarty Hall, PO Box 110300, 32611-0300 Gainesville, FL |
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Abstract: | Summary Sugarcane (Saccharum spp. hybrid cv. CP 84-1198) seeds were germinated on modified Murashige and Skoog (MS) basal medium alone or supplemented
with 2.3, 4.5, 11.3, 22.5, and 45.0 μM thidiazuron (TDZ), or 4.5, 13.6, 22.6, and 45.0 μM 2,4-dichlorophenoxyacetic acid (2,4-D), or 4.1, 12.4, 20.7, and 41.3, μM picloram. Both auxins delayed seed germination by approximately 5 d. Maximum germination was observed on MS medium supplemented
with 45.0 μM TDZ. Callus induction occurred for seed germinated on 2,4-D and picloram-containing media, but not on TDZ medium. The greatest
amount of callus (554±198mg per seed) was produced on 4.1 μM picloram. For shoot initiation, calluses were transferred to MS medium alone or supplemented with 2.5 μM TDZ. The highest number of shoots was recorded on TDZ medium from callus that had been obtained originally from media containing
either 4.1 or 12.4 μM picloram or 13.6 μM 2,4-D (∼500). All shoots developed roots and grew to maturity on medium with 24,6 μM indolebutyric acid. |
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Keywords: | embryogenic callus embryoids regeneration sugarcane seed |
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