Amine uptake in chloroplasts |
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| Authors: | R E Gaensslen R E McCarty |
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| Affiliation: | 1. Department of Environmental Analysis, Faculty of Chemistry, University of Gdańsk, ul. Wita Stwosza 63, PL-80-308 Gdańsk, Poland;2. BAM Federal Institute for Materials Research and Testing, Unter den Eichen 87, 12205, Berlin, Germany;3. Department of Organic Chemistry, Faculty of Chemistry, University of Gdańsk, ul. Wita Stwosza 63, PL-80-308 Gdańsk, Poland;1. Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia;2. Novosibirsk State University, Novosibirsk, Russia;3. Boreskov Institute of Catalysis, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia;4. Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia;5. FBRI State Research Centre of Virology and Biotechnology ‘Vector’, Koltsovo, Novosibirsk, Russia;1. The Faculty of Biomedical Sciences and Postgraduate Training, Medical University of Lodz, 4 Kosciuszki St, 90-419 Lodz, Poland;2. Laboratory of Molecular Virology and Biological Chemistry, Institute of Medical Biology, Polish Academy of Sciences, 106 Lodowa St, 93-232 Lodz, Poland;3. Department of Theoretical and Structural Chemistry, Faculty of Chemistry, University of Lodz, 163/165 Pomorska St, 90-236 Łódź, Poland;4. Screening Laboratory, Institute of Medical Biology, Polish Academy of Sciences, 106 Lodowa St, 93-232 Lodz, Poland |
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| Abstract: | Ethylamine uptake in illuminated chloroplasts, measured by a technique including microcentrifugation through silicone fluid layers, had properties similar to those of the light-dependent uptake of ammonia. The uptake of ethylamine was also found to be associated with adenosine triphosphate hydrolysis in the dark after activation of the magnesium-dependent adenosine triphosphatase in chloroplasts by light and dithiothreitol. From 1.3–1.7 μmoles of ethylamine were accumulated per μmole of adenosine triphosphate hydrolyzed, suggesting that 2 hydrogen ions are taken up per adenosine triphosphate split.The internal volume of chloroplasts illuminated in the presence of 4–6 mm ethylamine was two to three times greater than that of chloroplasts kept in the dark. At these concentrations of ethylamine, the internal concentration of ethylamine in illuminated chloroplasts was about ten times greater than the external concentration, whereas at 0.1 mm ethylamine, the concentration gradient was about 250-fold. These gradients in ethylamine concentration correspond to a pH differential across chloroplast membranes of 1 unit and 2.4 units, respectively.The uptake of radioactive chloride ion accompanied that of ethylamine. At concentrations of chloride exceeding 20 mm, about 1 chloride ion is transported into illuminated chloroplasts per molecule of ethylamine accumulated. |
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