Adenylate cyclase inhibition and GTPase stimulation by somatostatin in S49 lymphoma cyc- variants are prevented by islet-activating protein |
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Authors: | K Aktories G Schultz K H Jakobs |
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Institution: | 1. Nencki Institute of Experimental Biology, Department of Biochemistry of Nervous System and Muscle, Warsaw, 3 Pasteur Str. Poland;2. Institute of Protein Research, USSR Academy of Sciences, 142292 Poustchino, Moscow Region, USSR |
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Abstract: | cyc--Variants of S49 lymphoma cells are defective in the stimulatory guanine nucleotide site of the adenylate cyclase but contain an inhibitory site. Treatment of cyc- cells with islet-activating protein (IAP), which causes ADP-ribosylation of an Mr 40 000 polypeptide in cyc- membranes, abolishes adenylate cyclase inhibition by GTP and the peptide hormone, somatostatin, but not that induced by GTP gamma S. Furthermore, somatostatin-induced stimulation of GTP hydrolysis is lost. Thus, the data indicate that IAP interferes with the adenylate cyclase system by an action at the inhibitory guanine nucleotide site. |
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