The distribution of alpha-melanocyte stimulating hormone (alpha-MSH) in the central nervous system of the rat: an immunohistochemical study. II. Lower brain stem |
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Authors: | M Yamazoe S Shiosaka A Yagura Y Kawai T Shibasaki N Ling M Tohyama |
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Institution: | 1. Department of Neuroanatomy, Institute of Higher Nervous Activity, Osaka University Medical School 4-3-57 Nakanoshima Kitaku, Osaka (530), Japan;2. Department of Medicine, Tokyo Women''s Medical College, Tokyo, Japan;3. Laboratoires for Neuroendocrinology, The Salk Institute for Biological Studies, La Jolla, CA USA |
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Abstract: | The distribution of immunoreactive alpha-melanocyte stimulating hormone (alpha-MSHI) in the rat lower brain stem was examined by indirect immunofluorescence or peroxidase- anti-peroxidase immunohistochemical method using an antiserum against synthetic alpha-MSH. The results confirmed the presence of alpha-MSHI fibers in the midbrain central gray matter and parabrachial area, and demonstrated a much more extensive distribution of these fibers in various parts of the lower brain stem areas previously thought not contain alpha-MSHI fibers. In addition, the commissural nucleus was identified as a new alpha-MSHI neurons-containing site. No alpha-MSHI neurons were seen in other regions of the rat lower brain stem. |
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Keywords: | α-MSH Distribution Lower brain stem Immunocytochemistry |
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