Simultaneous production and partition of chitinase during growth of Serratia marcescens in an aqueous two-phase system |
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| Authors: | Jyh-Ping Chen Maw-Shyan Lee |
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| Institution: | (1) Department of Chemical Engineering, National Cheng Kung University, 701 Tainan, Taiwan, R.O.C.;(2) Present address: Department of Chemical Engineering, Chang Gung College of Medicine and Technology, 333 Kwei-San, Tao-Yuan, Taiwan, R.O.C. |
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| Abstract: | Summary Partition and production of the extracellular chitinase from Serratia marcescens were studied in PEG/dextran aqueous two-phase systems. The enzyme partitions into the bottom phase and the cells segregate into the top phase. The best system is 2% (w/v) PEG 20000 and 5% (w/v) dextran T500. The cell growth and enzyme production kinetics are similar in the aqueous two-phase system and in the polymer-free reference system. However, the maximum enzyme concentration in the former system is 1.5 times that in the latter one. |
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