镉胁迫下三个萝卜栽培种蛋白质变化的双向电泳比较研究

在0.5mmol·L^-1 Cd²⁺处理时,小五缨(XWY)的发芽率下降至92%,并且随着Cd²⁺浓度的增加,发芽率逐渐降低,在1和5mmol·L^-1 Cd²⁺浓度处理时,发芽率分别降至83%和67%。象牙白(XYB)则在5mmol·L^-1 Cd²⁺浓度时,发芽率下降。卫青(WQ)在0.05mmol·L^-1 Cd²⁺浓度时,发芽率已降至83%, 1和5mmol·L^-1时则下降至58%。幼苗生长也明显受Cd²⁺处理影响,在0.05mmol·L^-1 Cd²⁺处理时, 3种栽培萝卜的幼苗生长均受到明显影响,并且随Cd²⁺浓度的增加,生长受抑加重。从发芽率和幼苗生长两种实验结果看,卫青对Cd²⁺最为敏感。双向电泳结果表明, Cd²⁺处理后3种栽培萝卜幼苗中蛋白质组分有明显变化。小五缨中, 0.1 mmol·L^-1Cd²⁺处理后,有5个蛋白质点消失, 15个新的蛋白质点被诱导产生。象牙白中, 2个蛋白质点消失, 1个蛋白质点含量减少, 13个新的蛋白质点被诱导产生。卫青中, 12个新的蛋白质点诱导产生,但没有发现蛋白质点消失现象。Cd²⁺处理后, 3种栽培萝卜中,蛋白质合成的变化与幼苗生长受抑存在明显相关性,这一实验结果对于探讨萝卜Cd²⁺害的生化机理是有重要意义的。

Protein changes induced by cadmium stress in three radish cultivar seedlings:a comparison using two-dimensional gel electrophoresis

The germination rate was decreased to 92% when seeds were treated at 0.5 mmol · L-1 Cd2+ and was progressively decreased with higher Cd2+ 83% and 67% germination rates were observed respectively at 1 mmol · L-1 and 5 mmol · L-1 Cd2+ treatment in XWY, but the lower germination rate was observed with 5 mmol · L-1 Cd2+ treatment in XYB. The germination rate was decreased to 83%only at 0.05 mmol · L-1 Cd2+ and only 58% germination rate was observed when seeds were treated at 1 mmol · L-1 and 5 mmol · L-1 Cd2+ in WQ. The effects of Cd2+ on seedling growth were observed in three radish cultivars. Seedling growth damage was much more serious with higher Cd2+ concentration.WQ is the most sensitive to Cd2+ treatment according to the experiment results which were obtained by testing germination rate and seedling growth in three radish cultivars. 5 protein spots were disappeared and 15 new protein spots were induced in XWY seedling which were treated by 0.1 mmol · L-1 Cd2+,2 protein spots were disappeared and 1 protein spot was decreased in content, 13 new protein spots were induced in XYB, 12 new protein spots were induced in WQ. A close positive relationship was observed between protein spots change and inhibited seedling growth in the three radish cultivars which were treated by 0.1 mmol · L-1 Cd2+. It may be important for understanding biochemical mechanism of Cd2+ damage.