DNA recovery from ancient tissues: problems and perspectives |
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Authors: | P Francalacci |
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Institution: | (1) Istituto di Antropologia, Università di Sassari, Via Regina Margherita 15, 07100 Sassari, Italy |
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Abstract: | The recovery, amplification and sequencing of nucleic acids from ancient smaples opens new possibilities in many different
fields, such as anthropology, archeaology, population genetics, animal and plant evolutionary studies, and forensic medicine.
The sample processing for DNA extraction and PCR amplification represents the most delicate phase of ancient DNA analysis,
with a major impact on the reproducibility and reliability of the results. In this paper some extraction protocols are reviewed
and discussed, with particular reference to the removal of the inhibitory substances usually present in extract from ancient
tissues. The effect of contamination from extraneous DNA, a possible source of misleading results, is discussed and guidelines
to detect and circumvent the problem are given. |
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Keywords: | Ancient DNA analysis Methodology Molecular Evolution |
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