首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Identification of hemoglobin types contained in single chicken erythrocytes by fluorescent antibody technique
Authors:Koji Shimizu
Institution:Department of Morphology, Institute of Developmental Research, Aichi Prefecture Colony, Kasugai, Aichi 480-03, Japan
Abstract:It has been suggested that the switch in hemoglobin (Hb) types (from embryonic to adult) during chicken embryonic development is associated with the substitution of one erythroid cell line (“primitive”) for another (“definitive”). For the detection of two Hb types inside single erythroid cells, rabbit antibodies specific for embryonic and adult Hbs were prepared. Rabbit antibody specific for embryonic Hb cross-reacted only with embryonic major Hb components, while antibody specific for adult Hb did solely with adult minor Hb component. The antibodies were conjugated with fluorescein isothiocyanate. The conjugated antibodies were used for the fluorescent staining of blood smears of developing chicken embryos at different ages. Direct fluorescent antibody technique demonstrated that the major components of embryonic Hb and the minor component of adult Hb were not present within the same erythrocyte during chicken ontogenesis. It strongly suggested that embryonic-type Hb and adult-type Hb do not coexist within the same cell.
Keywords:
本文献已被 ScienceDirect 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号