Differential 51Cr uptake of human peripheral lymphocytes separated by density gradient electrophoresis |
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| Authors: | N. Catsimpoolas A.L. Griffith E.M. Skrabut C.D. Platsoucas C.R. Valeri |
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| Affiliation: | 1. Biophysics Laboratory, Department of Nutrition and Food Science, Massachusetts Institute of Technology, Cambridge, Mass. 02139 U.S.A.;2. Department of Hematology, Boston University Medical Center, Boston, Mass. 02118 U.S.A.;3. Naval Blood Research Laboratory, Chelsea, Mass. 02150 U.S.A. |
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| Abstract: | Human peripheral lymphocytes were labeled with 51Cr before or after separation by preparative density gradient electrophoresis. In both cases, wide variations in the distribution of 51Cr in the electrophoresed cells was observed. In general, there was significantly more 51Cr per cell in the high mobility fractions. These results suggest caution in the interpretation of cytotoxic assays where 51Cr-labeled lymphocytes are used as target cells and prompt further studies by different separation methods. |
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