Low‐intensity pulsed ultrasound induced enhanced adipogenesis of adipose‐derived stem cells |
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| Authors: | K. Ba Y. Fu X. Wei Y. Yue G. Li Y. Yao J. Chen X. Cai C. Liang Y. Ge Y. Lin |
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| Affiliation: | State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, , Chengdu, 610041 China |
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| Abstract: | Objective The aim of this study was to investigate effects of low‐intensity pulsed ultrasound (LIPUS) on differentiation of adipose‐derived stem cells (ASCs), in vitro. Materials and methods Murine ASCs were treated with LIPUS for either three or five days, immediately after adipogenic induction, or delayed for 2 days. Expression of adipogenic genes PPAR‐γ1, and APN, was examined by real‐time PCR. Immunofluorescence (IF) staining was performed to test for PPAR‐γ at the protein level. Results Our data revealed that specific patterns of LIPUS up‐regulated levels of both PPAR‐γ1 and APN mRNA, and PPAR‐γ protein. Conclusions In culture medium containing adipogenic reagents, LIPUS enhanced ASC adipogenesis. |
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