黑杨水培中营养液抑菌处理研究

水培条件下进行快速繁殖是大量生产黑杨种苗的途径之一 ,营养液的抑菌处理是水培的一个重要方面。该研究进行了二硫氰甲烷、中生菌素、敌磺钠、链霉素、青霉素等五种杀细菌剂和多菌灵、福美双、三唑酮等三种杀真菌剂的抑菌试验 ,结果表明 ,杀细菌剂对微生物生长的抑制率要高于杀真菌剂 ,二硫氰甲烷和中生菌素能较好地抑制营养液中微生物的生长 ,浓度分别为 2 0mg/L和 1 5mg/L时 ,抑制率分别为 75 .60 %和78 94%。杀真菌剂和杀细菌剂复配对营养液中微生物生长的抑制表现出较好的加和作用 ,其中以中生菌素(1 5mg/L)与福美双 (5mg/L)复配的效果最好 ,抑制率为 87.48%。

Antimicrobial treatment of nutrient solution in hydroponic cultivation of Populus nigra

Fast micro-propagation by hydroponic cultivation is one of the methods in large scale production of Populus nigra seedlings. Antimicrobial treatment of nutrient solution is an important aspect in hydroponic cultivation. Inhibition experiments on microbial growth were carried out by using five bactericides which were diisothiocyanatomethane, zhongshengmycin,fenaminosulf,streptomycin,penicillin,and three fungicides which were carbendazim,thiram and triadimefon. The results showed that the inhibition effects of bactericides against microorganisms in the nutrient solution were better than those of the fungicides. Of bactericides,both diisothiocyanatomethane and zhongshengmycin at their concentrations of 20 mg/L and 15 mg/L strongly inhibited the growth of the microorganisms which the corresponding inhibitory rate were 75.60% and 78.94% separately. It was found that microbial growth were inhibited to a great extent by the combination of one bactericide with one fungicide,and an additional effects of the agrochemicals were also observed. The inhibition rate of zhongshengmycin at 15 mg/L in combination with thiram at 5 mg/L was 87.48% which was the highest inhibition rate of all combination effects.