Redistribution of membrane-bound Ca2+ in neurolemma of leech Retzius-neurons during thermostimulation

Changes in electrical activity, plasma membrane and inner mitochondrial membrane potentials, as well as in the content of membrane-bound calcium and NADH in identified leech ganglion neurons during thermal stimulation were studied by microelectrode and microfluorimetric methods. It was shown that thermal stimulation of skin patches from different regions of leech body causes a reversible increase in the firing rate of pacemaker neurons (Rz neurons). A decrease in the content of membrane-bound calcium in the neurolemma and NADH content in Rz neuron mitochondria, and a depolarization of inner mitochondrial membrane were also observed.