Efficient biotransformation of herbicide diuron by bacterial strain <Emphasis Type="Italic">Micrococcus</Emphasis> sp. PS-1 |
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Authors: | Priyanka Sharma Adity Chopra Swaranjit Singh Cameotra C Raman Suri |
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Institution: | (1) Institute of Microbial Technology (CSIR), Sector 39-A, Chandigarh, 160036, India; |
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Abstract: | A Gram-positive, Micrococcus sp. strain PS-1 capable of utilizing phenylurea herbicide diuron as a sole carbon source at a high concentration (up to 250 ppm)
was isolated from diuron storage site by selective enrichment study. The taxonomic characterization with 16S rRNA gene sequencing
(1,477 bp) identified PS-1 as a member of Micrococcus sp. It was studied for the degradation of diuron and a range of its analogues (monuron, linuron, monolinuron, chlortoluron
and fenuron). The shake flasks experiments demonstrated fast degradation of diuron (up to 96% at 250 ppm within 30 h incubation)
with the addition of small quantity (0.01%) of non-ionic detergent. The relative degradation profile by the isolate was in
the order of fenuron > monuron > diuron > linuron > monolinuron > chlortoluron. Further, the biochemical characterization
of catabolic pathway by spectroscopic and chromatographic techniques demonstrated that the degradation proceeded via formation
of dealkylated metabolites to form 3,4-dichloroaniline (3,4-DCA). It was the major metabolite formed, associated with profound
increase in degradation kinetics in presence of appropriate additive. |
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