Comparative proteomic analysis of apomictic monosomic addition line of <Emphasis Type="Italic">Beta corolliflora</Emphasis> and <Emphasis Type="Italic">Beta vulgaris</Emphasis> L. in sugar beet |
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Authors: | Hong Zhu Ying-Dong Bi Li-Jie Yu De-Dong Guo Bai-Chen Wang |
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Institution: | (1) Department of Biology, College of Life and Environment Science, Harbin Normal University, 150080 Harbin, China;(2) Education Ministry, Key Laboratory of Forest Tree Genetic Improvement and Biotechnology, Northeast Forestry University, 150040 Harbin, People’s Republic of China;(3) Department of Biology, Heilongjiang University, 150080 Harbin, China |
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Abstract: | Apomixis refers to a process in which plants produce seed without fertilization through female syngamy that produces embryos
genetically identical to the maternal parent. In sugar beet, interspecific hybrids between diploid Beta vulgaris and tetraploid Beta corolliflora were established and monosomic addition line M14 was selected because of the apomictic phenotype. By using two-dimensional
electrophoresis gels we identified the proteins which were differently expressed between the M14 and B. vulgaris. A total of 27 protein spots which varied expressed between lines were isolated and successfully identified with MALDI-TOF
MS. Among them five protein spots were found to be only presented in M14 and two protein spots only expressed in Beta. According
to their functional annotations described in Swissprot database, these proteins were, respectively, involved in important
biological pathways, such as cell division, functionally classified using the KEGG functional classification system. The result
may be useful for us to better understand the genetic mechanism of apomixes. |
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Keywords: | |
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