Bioaccumulation of Arsenic in recombinant Escherichia coli expressing human metallothionein |
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| Authors: | Yu-Jie Su Jian-Qun Lin Jian-Qiang Lin Dong-Hui Hao |
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| Institution: | (1) Department of Chemical and Biomolecular Engineering (BK21 Program), Metabolic and Biomolecular Engineering National Research Laboratory, KAIST, 335 Gwahangno, Daejeon, 305-701, Republic of Korea;(2) Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, 335 Gwahangno, Daejeon, 305-701, Republic of Korea;(3) Department of Bio and Brain Engineering, BioProcess Engineering Research Center and Bioinformatics Research Center, KAIST, 335 Gwahangno, Daejeon, 305-701, Republic of Korea;(4) Department of Biological Sciences, KAIST, 335 Gwahangno, Daejeon, 305-701, Republic of Korea; |
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| Abstract: | The recombinant Escherichia coli (E. coli) expressing human hepatic metallothionein_IA (hMT_IA) was constructed for bioaccumulation of Arsenic (As). The gene sequence
of hMT_IA was modified for codon preference of E. coli and synthesized using chemical method. The vector of pGEX_4T_1 was used and hMT_IA was expressed as the fusion protein with
glutathione S-transferase (GST) tag. The bioaccumulation capability of arsenite compounds As(III) of the recombinant E. coli increased more than 3-fold from 76.3 to 319.6 μg/g dry cells compared with the control. The conditions of 50 μM of As(III)
and low pHs were optimal for As(III) bioaccumulation. The heavy metals of Cd, Hg, and Zn inhibited As(III) bioaccumulation.
The bioaccumulation reached 70% of the saturated value within 1 h. The recombinant E. coli will be useful in bioremediation of arsenic or other kinds of heavy metal contaminated water. |
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