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| 红树植物秋茄叶片双向电泳技术体系的建立及优化 | |
| 引用本文： | 于冯,郑春芳,施孟如,刘伟成,仇建标,冀德伟,黄丽,陈少波.红树植物秋茄叶片双向电泳技术体系的建立及优化[J].热带亚热带植物学报,2011,19(6):519-523. |
| 作者姓名： | 于冯 郑春芳 施孟如 刘伟成 仇建标 冀德伟 黄丽 陈少波 |
| 作者单位： | 1. 温州医学院生命科学学院,浙江温州325035;浙江省海洋水产养殖研究所,浙江省近岸水域生物资源开发与保护重点实验室,浙江温州325005 2. 浙江省海洋水产养殖研究所,浙江省近岸水域生物资源开发与保护重点实验室,浙江温州325005 3. 温州医学院生命科学学院,浙江温州,325035 |
| 基金项目： | 国家海洋公益性行业科研专项经费项目(200805072; 201005012);浙江省近岸水域生物资源开发与保护重点实验室开放课题基金项目(2010F30003); 浙江省科技厅创新团队建设与人才培养项目(2009F20009); 浙江省海洋与渔业项目"高纬度红树林生态恢复关键技术应用示范"资助 |
| 摘 要： | 对适用于秋茄(Kandelia candel)叶片蛋白质组学研究的双向电泳技术体系进行了优化.结果表明,采用酚抽提法提取的蛋白质浓度较低,约1.7 μg μL-1,SDS-PAGE电泳后几乎无条带;而采用改良TCA -丙酮沉淀法可显著提高提取液中蛋白质的含量,可达12.4 μgμL-1.秋茄叶片蛋白质主要分布在pH4～... |
| 关 键 词： | 秋茄 蛋白质 提取 双向电泳(2-DE) 技术优化 |
| 收稿时间： | 3/7/2011 12:00:00 AM |
| 修稿时间： | 2011/8/17 0:00:00 |
Establishment and Optimization of 2 - DE Technique System in Leaf Proteome of Kandelia candel |
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| YU Feng,ZHENG Chun-fang,SHI Meng-ru,LIU Wei-cheng,QIU Jian-biao,JI De-wei,HUANG Li and CHEN Shao-bo.Establishment and Optimization of 2 - DE Technique System in Leaf Proteome of Kandelia candel[J].Journal of Tropical and Subtropical Botany,2011,19(6):519-523. | |
| Authors: | YU Feng ZHENG Chun-fang SHI Meng-ru LIU Wei-cheng QIU Jian-biao JI De-wei HUANG Li and CHEN Shao-bo |
| Institution: | 1. College of Life Sciences, Wenzhou Medical College, China; 2. Zhejiang Mariculture Research Institute, Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-resource, China;Zhejiang Mariculture Research Institute, Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-resource, China;College of Life Sciences, Wenzhou Medical College, China;Zhejiang Mariculture Research Institute, Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-resource, China;Zhejiang Mariculture Research Institute, Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-resource, China;Zhejiang Mariculture Research Institute, Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-resource, China;Zhejiang Mariculture Research Institute, Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-resource, China;Zhejiang Mariculture Research Institute, Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-resource, China |
| Abstract: | |
| Keywords: | Kandelia candel Protein Extraction Two-dimensional electrophoresis (2-DE) Optimization |
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