Association of metalloproteinases,tissue inhibitors of matrix metalloproteinases,and proteoglycans with development,aging, and osteoarthritis processes in mouse temporomandibular joint |
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Authors: | Amira?Gepstein,Gil?Arbel,Israel?Blumenfeld,Micha?Peled,Erella?Livne author-information" > author-information__contact u-icon-before" > mailto:Livne@tx.technion.ac.il" title=" Livne@tx.technion.ac.il" itemprop=" email" data-track=" click" data-track-action=" Email author" data-track-label=" " >Email author |
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Affiliation: | (1) Department of Anatomy and Cell Biology, Bruce Rappaport Faculty of Medicine, Technion-Israel Institute ofTechnology, POB 9649, 31096 Haifa, Israel;(2) Department of Maxillofacial Surgery, Rambam Medical Center, Haifa, Israel |
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Abstract: | The temporomandibular joint (TMJ) is an important growth and articulation center in the craniofacial complex. In aging it develops spontaneous degenerative osteoarthritic (OA) lesions. Metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPS) play key roles in extracellular matrix remodeling and degradation. Gelatinase activities and immunohistochemical localization of MMP-2, -3, -8, -9, and -13 and TIMP-1 and -2 were examined in mandibular condyle cartilage of neonatal mice up to 18 months old. The most intense immunostaining for all enzymes and TIMPs and the peak of gelatinase activities were found in animals in the stages of early growth (1 week to 3 months) followed by a decrease during maturation and aging. However, clusters of positively immunoreactive chondrocytes were detected in cartilages of old animals displaying OA lesions. Positive safranin-O staining, indicative of sulfated proteoglycans (PGs), was prominent in the TMJ of newborn mice up to 3 months old followed by reduction during maturation and aging, except in regions displaying OA lesions. Temporal codistribution of PGs, MMPs, and TIMPs during skeletal maturation reflected an active growth phase, whereas their reduction coincided with the more quiescent articulating and maintenance phase in the joint cartilage. Osteoarthritic lesions were associated with both increased PG synthesis and MMP immunoreactivity, indicating limited repair activity during initial stages of osteoarthritis. |
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Keywords: | Osteoarthritis Articular cartilage Aging MMPs Proteoglycans Mice |
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