Simplified production and concentration of HIV-1-based lentiviral vectors using HYPERFlask vessels and anion exchange membrane chromatography |
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| Authors: | Robert H Kutner Sharon Puthli Michael P Marino Jakob Reiser |
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| Affiliation: | (1) Gene Therapy Program, Vector Core, Louisiana State University Health Sciences Center, New Orleans, LA, USA;(2) Division of Cellular and Gene Therapies, Office of Cellular, Tissue and Gene Therapies, US Food and Drug Administration, Center for Biologics Evaluation and Research, Bethesda, MD, USA |
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| Abstract: | Background During the past twelve years, lentiviral (LV) vectors have emerged as valuable tools for transgene delivery because of their ability to transduce nondividing cells and their capacity to sustain long-term transgene expression in target cells in vitro and in vivo. However, despite significant progress, the production and concentration of high-titer, high-quality LV vector stocks is still cumbersome and costly. |
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