SIPA1 boosts migration and proliferation,and blocks apoptosis of glioma by activating the phosphorylation of the FAK signaling pathway |
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| Authors: | Yuan Du Shenglan Li Tong Zhou Jing Zhao Jiguang Liu |
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| Affiliation: | 1. Jiamusi University, School of Basic Medicine Science, Jiamusi, China ; 2. Central Hospital of Jamusi City, Jiamusi, China ; 3. Capital Medical University, Beijing Tiantan Hospital, Department of Neuro-oncology, Cancer center, Beijing, China ; 4. Jiamusi University, School of Pharmacy, Jiamusi, China ; 5. First Affiliated Hospital of Jiamusi University, Clinical Laboratory, Jiamusi, China ; 6. Jiamusi University, School of Stomatology, Jiamusi, China |
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| Abstract: | BackgroundWe aimed to analyze the regulatory effects of SIPA1 (signal-induced proliferation-associated protein 1) on glioma progression and the dominant signaling pathway.MethodsDifferential level of SIPA1 in glioma and normal tissues and cells was determined. Migratory, proliferative, apoptotic and cell cycle progression changes in A172 cells with overexpression or knockdown of SIPA1 were examined. Finally, protein levels of phosphorylated FAKs in A172 cells intervened by SIPA1, and the FAK inhibitor PF562271 were detected.ResultsSIPA1 was upregulated in glioma cases. Knock-down of SIPA1 reduced migratory and proliferative rates of glioma cells, increased apoptotic cell rate, and declined cell ratio in the S phase. The knockdown of SIPA1 also downregulated cell cycle proteins. In addition, SIPA1 upregulated phosphorylated FAKs in A172 cells and thus boosted malignant phenotypes of glioma.ConclusionsSIPA1 is upregulated in glioma that boosts migratory and proliferative potentials of glioma cells by activating the phosphorylation of the FAK signaling pathway. |
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| Keywords: | SIPA1 FAK phosphorylation glioma |
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