Development of in vitro procedures for regeneration of petiole and leaf expiants and production of protoplast-derived callus in Tanacetum vulgare L. (Tansy) |
| |
Authors: | Marjo Keskitalo Timo Kanerva Eija Pehu |
| |
Affiliation: | (1) Department of Plant Production, University of Helsinki, P.O. Box 27, FIN-00014, Finland |
| |
Abstract: | Tanacetum vulgare (Tansy) was established in vitro on Murashige and Skoog (MS) medium supplemented with naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP) using shoot tips and embryos. From petiole expiants 93% formed callus, and 27% produced shoots on MS medium containing 4.5 mg l-1 NAA and BAP. NAA alone induced root formation from leaf expiants. Up to 7 ×106 viable protoplasts were obtained by macerating 1 g of leaves in 0.5 % Macerozyme R-10, 1.0% Cellulase R10, and 1.0% Cellulysin. Cell division was observed 3–4 days after protoplast isolation at the optimum plating density of 0.2-0.4×106 cells ml-1. A total of 350 protoplast-derived calluses were produced on which nodules with meristematic zones developed. Roots regenerated on MS medium supplemented with BAP 3.0 mg 1-1, NAA 2.0 mg l-1, and 250 mg l-1 casein hydrolysate, however no shoots have been obtained yet.Abbreviations BAP 6-benzylaminopurine - CH casein enzymatic hydrolysate - 2.4 D dichlorophenoxyacetic acid - FDA fluorescein diacetate - GA3 gibberellic acid - IBA indole butyric acid - IPA 6-dimethylallylamino purine - KIN Kinetin - MS Murashige and Skoog medium - NAA -naphthaleneacetic acid |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|