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| 无胶筛分毛细管电泳在分析苹果发育时期转变蛋白质变化规律中的应用 | |
| 引用本文： | 田义,张新忠,张志宏,丛佩华,康国栋.无胶筛分毛细管电泳在分析苹果发育时期转变蛋白质变化规律中的应用[J].植物学通报,2008,25(5):585-590. |
| 作者姓名： | 田义 张新忠 张志宏 丛佩华 康国栋 |
| 作者单位： | 1. 中国农业科学院果树研究所,兴城,125100 2. 中国农业大学,北京,100094 3. 沈阳农业大学,沈阳,110161 |
| 基金项目： | 国家高技术研究发展计划(863计划)，河北省自然科学基金 |
| 摘 要： | 对无胶筛分毛细管电泳体系的筛分介质浓度、缓冲体系的离子强度、分离电压、进样量及温度等条件进行了优化，最终确定最佳分离条件为7.5% Dextran，7.5%丙三醇，0.15mol·L^-1 TB，0.1%SDS，分离电压23.5kV，进样量1.5psi×90秒，温度25℃。应用此方法对金冠×红玉苹果杂种后代韧皮部蛋白质进行测定，得到了多个与阶段转变相关的蛋白质，在韧皮部50节上，有分子量为5.0kDa的特异蛋白质出现，分子量为24.5kDa的蛋白质含量在阶段转变过程中明显升高。 |
| 关 键 词： | 苹果 无胶筛分毛细管电泳 阶段转变 蛋白质 |
Analysis of Non-gel Sieving Capillary Electrophoresis in Protein Changes Pattern in Development Phase Shift in Apple |
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| Yi Tian,Xinzhong Zhang,Zhihong Zhang,Peihua Cong,Guodong Kang.Analysis of Non-gel Sieving Capillary Electrophoresis in Protein Changes Pattern in Development Phase Shift in Apple[J].Chinese Bulletin of Botany,2008,25(5):585-590. | |
| Authors: | Yi Tian Xinzhong Zhang Zhihong Zhang Peihua Cong Guodong Kang |
| Institution: | Yi Tian, Xinzhong Zhang, Zhihong Zhang, Peihua Cong, Guodong Kang(1.Research Institute of Pomology, The Chinese Academy of Agricultural Sciences, Xingcheng 125100, China;2.China Agricultural University, Beijing 100094, China; 3. Shenyang Agricultural University, Shenyang 110161, China) |
| Abstract: | Protein markers were used with optimal electrophoresis conditions in analysis of proteins of phase change in apple. Optimal conditions for non-gel sieving capillary electrophoresis （NGSCE） were 0.15 mol·L^-1 TB, 0.1% SDS, 7.5% Dextran, 7.5% glycerol, 23.5 kV separation voltage and 1.5 psix90 s injection volume. This condition for NGSCE was used to separate the phloem proteins in apple hybrid seedlings. A 5.0-kDa protein appeared above the 50th node, and the content of a 24.5-kDa protein increased sharply during phase change. The result was valuable for study of apple phase change. |
| Keywords: | apple non-gel sieving capillary electrophoresis phase change protein |
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