Omp85 genosensor for detection of human brain bacterial meningitis |
| |
Authors: | Sandip Kumar Dash Minakshi Sharma Shashi Khare Ashok Kumar |
| |
Institution: | 1. CSIR-Institute of Genomics and Integrative Biology, Mall Road, Delhi, 110007, India 2. Department of Zoology, M. D. University, Rohtak, 124001, Haryana, India 3. National Centre for Disease Control, Sham Nath Marg, Delhi, 110054, India
|
| |
Abstract: | The 5′-thiolated DNA probe based on specific virulence gene, Omp85, was immobilized onto a screen-printed gold electrode followed by hybridization with 6–100 ng/6 μl (5.9 × 105–9.3 × 106 c.f.u.) of Neisseria meningitidis single stranded genomic DNA (ssG-DNA) for 10 min at 25 °C from the cerebrospinal fluid (CSF) of a meningitis patient. The Omp85 genosensor can detect as little as 6 ng ssG-DNA in 6 μl CSF of a human brain meningitis patient in 30 min including a response time of 1 min by cyclic voltammetry, differential pulse voltammetry (DPV) and electrochemical impedance. The sensitivity of the genosensor electrode was 2.6(μA/cm2)/ng using DPV with regression coefficient (R2) 0.954. The genosensor was characterized using Fourier transform infrared spectroscopy and atomic force microscopy. Omp85 genosensor was stable for 12 months at 4 °C with 12 % loss in DPV current. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|