Immunocytochemical localization of methionine enkephalin: preliminary observations. |
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Authors: | S J Watson H Akil S Sullivan J D Barchas |
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Affiliation: | Nancy Pritzker Laboratory of Behavioral Neurochemistry Department of Psychiatry and Behavioral Sciences Stanford University School of Medicine Stanford, California 94305, USA |
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Abstract: | Antiserum directed against methionine enkephalin (metenkephalin) was used to determine its anatomical distribution in rat brain. Cross reactivity of that antiserum was not detectable against leucine enkephalin (leu-enkephalin), β-lipotropin (β-LPH), β-endorphin or assorted peptide fragments of met-enkephalin; alpha-endorphin was 370 times less active than met-enkephalin. The localization of met-enkephalin was carried out in the presence of excess leu-enkephalin and yet could be blocked with equal amounts of met-enkephalin. Met-enkephalin was detected in several structures in the spinal cord, medulla, pons, mesencephalon, diencephalon and telencephalon. No met-enkephalin was detected in cerebellum or cerebral cortex. |
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