Localization of ribosomal cistrons in metaphase chromosomes of Vicia faba (L.) |
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| Authors: | W Scheuermann Marita Knlmann |
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| Institution: | 1. Institut für Strahlenbotanik der Gesellschaft für Strahlen und Umweltforschung m.b.H., München, Hannover, BRD;2. Institut für Biophysik der Technischen Universität Hannover, BRD |
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| Abstract: | Tritiated ribosomal RNA (rRNA) was prepared from the roots of Vicia faba after incubation in 3H-uridine. Separation of the nucleic acids by MAK chromatography yielded fractions of specific activity of 4–5 × 105 dpm/μg. 4 + 5S, 18S and 25S RNA fractions were used for cytological hybridization on squash preparations of Vicia faba root tip meristems. Autoradiographs of the 18S and 25S RNA preparations exhibited a clear labelling in the secondary constriction of the satellite (SAT) chromosomes after exposition times of 28 weeks. |
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