Phospholipid synthesis and degradation during the life-cycle of P815Y mast cells synchronized with excess of thymidine |
| |
Authors: | J. J. M. Bergeron A. M. H. Warmsley C. A. Pasternak |
| |
Affiliation: | Department of Biochemistry, University of Oxford, Oxford OX1 3QU, U.K. |
| |
Abstract: | 1. P815Y cells synchronized with excess of thymidine incorporate choline, proline and uridine throughout the cell cycle; the rate increases two- to four-fold during the S phase, when thymidine incorporation increases more than 15-fold. 2. Choline incorporated at any stage of the cell cycle turns over in a biphasic manner; stable and unstable components are each labelled maximally during the S phase. Total phospholipid also doubles predominantly during the S phase. 3. It is concluded that, despite turnover, choline incorporation is a useful measure of net phospholipid formation during the cell cycle. |
| |
Keywords: | |
|
|