Induction of both cytochromes P-450 and P-448 by 2,3',4,4',5-pentabromobiphenyl, a component of fireMaster |
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| Authors: | L W Robertson A Parkinson S Safe |
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| Affiliation: | School of Public Health, The University of Michigan, Ann Arbor, MI 48109 USA;Guelph-Waterloo Centre for Graduate Work in Chemistry, Department of Chemistry, Unviersity of Guelph, Guelph, Ontario N1G 2W1 Canada |
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| Abstract: | The synthesis and purification of a component of fireMaster BP-6 and fireMaster FF-1, 2,3′,4,4′,5-pentabromobiphenyl, is described. The compound was found to be a potent inducer of liver microsomal drug-metabolizing enzymes in the rat, enhancing those enzymic activities induced by both phenobarbitone and 3-methylcholanthrene (i.e. cytochromes P-450 and P-448). The pentabromobiphenyl enhanced the activities of benzo[a]pyrene hydroxylase, dimethylamino-antipyrine N-demethylase and NADPH-cytochrome c reductase. The hepatic cytochromes b5 and P-450 were increased and the Soret peak maximum of the latter was shifted to 448.5 nm. The relative peak intensities and spectral shifts for the ethylisocyanide-binding difference spectra confirmed the mixed induction characteristics of 2,3′,4,4′,5-pentabromobiphenyl. |
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| Keywords: | PBBs polybrominated biphenyls PB phenobarbitone MC 3-methylcholanthrene NMR nuclear magnetic resonance PBBp 2,3′,4,4′,5-pentabromobiphenyl GC gas chromatographic TBBp 2,3′,4′,5-tetrabromobiphenyl B[a]P benzo[a]pyrene EIC ethylisocyanide DMAP 4-dimethylaminoantipyrine CO carbon monoxide |
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