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Restoration of virulence of Vir region mutants of Agrobacterium tumefaciens strain B6S3 by coinfection with normal and mutant Agrobacterium strains |
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| Authors: | L. Otten H. De Greve J. Leemans R. Hain P. Hooykaas J. Schell |
| Affiliation: | (1) Max-Planck-Institut für Züchtungsforschung, D-5000 Köln 30, Federal Republic of Germany;(2) Laboratorium Genetische Virologie, Vrije Universiteit, Brussel, Belgium;(3) Department of Biochemistry, State University of Leiden, Leiden, The Netherlands |
| Abstract: | Summary Three avirulent Tn7 insertion mutants mapping in the vir E region of the Agrobacterium tumefaciens plasmid pTiB6S3 regain virulence by co-infection with several wildtype strains and with a number of strains carrying mutations in other regions of the Ti plasmid. This finding indicates that during tumour induction normal Agrobacterium strains produce a diffusable factor required for transformation and might allow the isolation of such a factor. |
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