Genetic requirements for the expression of benzylamine dehydrogenase activity in Pseudomonas putida |
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| Authors: | Megharaj Mallavarapu,Isabel Mö hler,Matthias Krü ger,Morris M Hosseini,Frank Bartels,Kenneth N Timmis,reas Holtel |
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| Affiliation: | GBF-National Centre for Biotechnology, Mascheroder Weg 1, 38124 Braunschweig, Germany;Department of Soil Sciences, University of Adelaide, Waite Campus, Glen Osmond, SA 5064, Australia;Centre for Biomaterials, University of Toronto, Toronto, Ont. M5S 1A1, Canada |
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| Abstract: | Pseudomonas putida grows on benzylamine as a sole source of carbon/energy and nitrogen. Synthesis of an inducible benzylamine dehydrogenase (BMDH) depends on the specific RNA polymerase sigma factor σ54 and is subject to carbon source-dependent inhibition. The presence of TOL plasmid pWW0 harboring the genetic information for the catabolism of toluene exerts strong inhibition of induction of BMDH activity. |
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| Keywords: | Pseudomonas putida Benzylamine dehydrogenase σ54 Catabolite repression TOL plasmid |
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