Analysis of pigmentation in individual cultured plant cells using an image processing system |
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Authors: | Kazuhiko Miyanaga Minoru Seki Shintaro Furusaki |
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Institution: | (1) Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, Tokyo, , Japan;(2) Department of Chemical Systems and Engineering, Graduate School of Engineering, Kyushu University, Kyushu, , Japan |
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Abstract: | Anthocyanin accumulation in strawberry (Fragaria ananassa) cells cultured on a solid medium was monitored using an image-processing system that did not require direct sampling or destruction of the cells. Because of the intercellular heterogeneity of secondary metabolite production in plant cell cultures, the maximum metabolite concentration in individual cells is often more than 10 times higher than that of the average concentration. An image-processing based method enabled the growth and the pigmentation behavior of individual cells to be traced. Changes in the time courses of the anthocyanin content of individual cells differed from each other, although the average anthocyanin contents increased gradually with time in a batch culture. However, these various changing patterns in the anthocyanin content of each cell were independent of the cell cycle. In addition, image analysis revealed that the two cells just after cell division were almost identical to each other both in size and anthocyanin content. The proposed method which uses an image-processing system provides a useful tool for analyzing the secondary metabolism in individual cultured plant cells. |
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Keywords: | anthocyanin cell division image analysis pigmentation |
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