Application of immobilized metal affinity chromatography in proteomics |
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Authors: | Sun Xuesong Chiu Jen-Fu He Qing-Yu |
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Institution: | Department of Chemistry, University of Hong Kong, Pokfulam, Hong Kong. sunxs@hkusua.hku.hk |
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Abstract: | It has been proved that the progress of proteomics is mostly determined by the development of advanced and sensitive protein separation technologies. Immobilized metal affinity chromatography (IMAC) is a powerful protein fractionation method used to enrich metal-associated proteins and peptides. In proteomics, IMAC has been widely employed as a prefractionation method to increase the resolution in protein separation. The combination of IMAC with other protein analytical technologies has been successfully utilized to characterize metalloproteome and post-translational modifications. In the near future, newly developed IMAC integrated with other proteomic methods will greatly contribute to the revolution of expression, cell-mapping and structural proteomics. |
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