Binding of antibromelain monomeric Fab' improves the stability of stem bromelain against inactivation |
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| Authors: | Gupta Pawan Khan Rizwan H Saleemuddin M |
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| Affiliation: | Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Uttar Pradesh, India. |
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| Abstract: | Antienzyme polyclonal antibodies against stem bromelain were raised in male albino rabbits and the Fab' monomers isolated from the IgG of the immune sera. Incubation of bromelain with the Fab' resulted in binding and gel filtration of the resulting complex suggested a 1:1 stoichiometry. Complexing with the Fab' resulted in significant stabilization of bromelain against thermal inactivation and alkaline pH. |
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