1. Departamento de Biología Celular, Facultad de Ciencias Biológicas, Center for Advanced Microscopy, CMA BIO BIO, Universidad de Concepción, , Concepción, Chile;2. Hospital Regional Guillermo Grant Benavente, , Concepción, Chile;3. Facultad de Medicina, Universidad de la Frontera, , Temuco, Chile;4. Departamento de Fisiología, Pontificia Universidad Católica de Chile, , Santiago, Chile;5. Department of Urology, Roswell Park Cancer Institute, , Buffalo, New York, USA
Abstract:
In vitro and in vivo studies suggest that the basolateral membrane of choroid plexus cells, which is in contact with blood vessels, is involved in the uptake of the reduced form of vitamin C, ascorbic acid (AA), through the sodium‐vitamin C cotransporter, (SVCT2). Moreover, very low levels of vitamin C were observed in the brains of SVCT2‐null mice. The oxidized form of vitamin C, dehydroascorbic acid (DHA), is incorporated through the facilitative glucose transporters (GLUTs). In this study, the contribution of SVCT2 and GLUT1 to vitamin C uptake in human choroid plexus papilloma (HCPP) cells in culture was examined. Both the functional activity and the kinetic parameters of GLUT1 and SVCT2 in cells isolated from HCPP were observed. Finally, DHA uptake by GLUT1 in choroid plexus cells was assessed in the presence of phorbol‐12‐myristate‐13‐acetate (PMA)‐activated human neutrophils. A marked increase in vitamin C uptake by choroid plexus cells was observed that was associated with superoxide generation and vitamin C oxidation (bystander effect). Thus, vitamin C can be incorporated by epithelial choroid plexus papilloma cells using the basolateral polarization of SVCT2 and GLUT1. This mechanism may be amplified with neutrophil infiltration (inflammation) of choroid plexus tumors.
