Detection and Molecular Variability of Apple Stem Grooving Virus in Shaanxi,China |
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Authors: | Huawei Zhang Honghong Jiao Yunfeng Wu |
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Institution: | State Key Laboratory of Crop Stress Biology for Arid Areas, Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau, Ministry of Agriculture, Key Laboratory of Plant Protection Resources and Pest Management, Ministry of Education, College of Plant Protection, Northwest A&F University, , Yangling, 712100 Shaanxi, China |
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Abstract: | Apple stem grooving virus (ASGV) is one of the economically important latent viruses that are distributed in apple production areas worldwide. The presence of ASGV in apple trees was studied by serological assay and molecular biology methods. A total of 550 apple leaf samples from 14 different areas in Shaanxi were tested by DAS‐ELISA, and the results revealed an ASGV infection level of 55%. Those samples were also examined by RT‐PCR, and an infection level of 67% was found. Fourteen complete coat protein gene sequences of ASGV were obtained; phylogenetic analysis revealed that these 14 sequences separated into two clusters regardless of the geographic origin or host plants. To our knowledge, this is the first report of molecular variability analysis of ASGV in apple trees in China. |
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Keywords: |
Apple stem grooving virus
coat protein gene multiple alignment phylogenetic analysis China |
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