Circulating plasma serine208‐phosphorylated troponin T levels are indicator of cardiac dysfunction |
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Authors: | Emilie Dubois‐Deruy Aude Belliard Paul Mulder Maggy Chwastyniak Olivia Beseme Jean‐Paul Henry Christian Thuillez Philippe Amouyel Vincent Richard Florence Pinet |
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Institution: | 1. INSERM, U744, , Lille, France;2. Institut Pasteur de Lille, , Lille, France;3. University of Lille 2, IFR141, , Lille, France;4. INSERM, U1096, Institute for Biomedical Research, IFRMP 23, Rouen University Hospital, , Rouen, France;5. Centre Hospitalier Regional et Universitaire, Cardiologic hospital, , Lille, France |
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Abstract: | Heart failure (HF) following myocardial infarction (MI) is characterized by progressive alterations of left ventricular (LV) structure and function, named LV remodelling. Although several risk factors such as infarct size have been identified, HF remains difficult to predict in clinical practice. Recently, using phosphoproteomic technology, we found that serine208‐phosphorylated troponin T (P‐Ser208‐TnT) decreases in LV of HF rats. Our aim was to determine the performance of P‐Ser208‐TnT as plasma biomarker of HF compared to conventional cardiac biomarkers such as B‐type natriuretic peptide (BNP), cardiac troponin I (cTnI), C‐reactive protein (CRP) or tissue inhibitor of metalloproteinase I (TIMP‐1) measured by x‐MAP technology, as well as its capacity to reflect a pharmacological improvement of HF. We observed a significant increase of BNP, TnT and cTnI levels and a significant decrease of P‐Ser208‐TnT and TIMP‐1 in the plasma of 2‐month‐MI rats compared with control rats with no modulation of CRP level. Circulating levels of P‐Ser208‐TnT were shown to be associated with most of the echocardiographic and haemodynamic parameters of cardiac function. We verified that the decrease of P‐Ser208‐TnT was not because of an excess of phosphatase activity in plasma of HF rats. Two‐month‐MI rats treated with the heart rate reducing agent ivabradine had improved LV function and increased plasma levels of P‐Ser208‐TnT. Thus, circulating phosphorylated troponin T is a highly sensitive biological indicator of cardiac dysfunction and has the potentiality of a new biomarker of HF post‐MI, and of a surrogate marker for the efficacy of a successful treatment of HF. |
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Keywords: | Rat myocardial infarction plasma biomarker phosphorylated troponin T ivabradine heart failure |
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