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STED microscopy of living cells – new frontiers in membrane and neurobiology |
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| Authors: | Christian Eggeling Katrin I. Willig Francisco J. Barrantes |
| Affiliation: | 1. Department of Nanobiophotonics, Max Planck Institute for Biophysical Chemistry, , G?ttingen, Germany;2. Weatherall Institute of Molecular Medicine, University of Oxford, , Oxford, UK;3. Laboratory of Molecular Neurobiology, Institute of Biomedical Research (BIOMED) UCA–CONICET, Catholic University of Argentina, , Buenos Aires, Argentina |
| Abstract: | Recent developments in fluorescence far‐field microscopy such as STED microscopy have accomplished observation of the living cell with a spatial resolution far below the diffraction limit. Here, we briefly review the current approaches to super‐resolution optical microscopy and present the implementation of STED microscopy for novel insights into live cell mechanisms, with a focus on neurobiology and plasma membrane dynamics. |
| Keywords: | fluorescence imaging membrane dynamics STED nanoscopy |